Convergent gene expression programs in human and mouse NOTCH1-induced T-ALL cells on HES1 inactivation. (A) Heat map representation of the top differentially expressed genes between Hes1 conditional knockout T-ALL (ΔE-NOTCH1 Rosa26 Cre-ERT2 Hes1^flox/flox) cells treated with vehicle only (ETOH) or tamoxifen (4-OH TMX) in vitro. The scale bar shows color-coded differential expression, with red indicating higher levels and blue indicating lower levels of expression. (B) GSEA enrichment plot and top leading-edge genes upregulated after Hes1 knockout compared with the transcriptional signature associated with HES1 knockdown in CUTLL1 cells. (C) GSEA enrichment plot and top leading-edge genes upregulated after GSI treatment of NOTCH1-induced murine T-ALL cells compared with the transcriptional signature induced by tamoxifen treatment in Hes1 conditional knockout leukemia cells (Hes1 KO).