Cytotoxicity of circulating γδ T cells. (A) CD107a expression in gated γδ⁺Vδ1⁺ and γδ⁺Vδ2⁺ T cells of PBMCs from patients cultured with AML blasts untreated (medium) or ZOL sensitized was investigated by flow cytometry. (B) One representative experiment using PBMCs obtained from patient 7. (C) Flow cytometric expression of CD107a in gated γδ⁺Vδ1⁺ and Vδ2⁺ T cells in patients’ PBMCs upon coculture with either BCP-ALL or T-ALL blasts, either untreated (medium) or ZOL sensitized. (D) CD107a expression in gated CD3⁺γδ⁺Vδ1⁺ cells from PBMCs collected from patients who did experience CMV reactivation (n = 10) and those who did not (n = 10) was evaluated. Pooled results are shown. Whisker lines represent the highest and lowest values; horizontal lines represent median values. One representative experiment performed with PBMCs from patients 18 and 9 is shown in dot plots in the right.