Gal3BP and gal3 at the lumen/IVC interface. Non-VT images: Top raw (A-D); VT images 48 hours after thrombosis was initiated from an area where there was not thrombus/vein wall contact, middle raw (E-H); and VT images 48 hours after thrombosis was initiated from an area where there was thrombus/vein wall fusion, bottom raw (I-L). Note that vein wall gal3 and gal3bp are abundant in areas where the thrombus is not contacting the vein wall (F-G), but disappear in areas where the thrombus contacts the vein wall (J-K). Red blood cell fluorescence is predominately nonspecific autofluorescence. The IVC lumen is indicated with an asterisk in DAPI images. White arrows indicate WBCs. (A,E,I) Nuclear DAPI staining. (B,F,J) Gal3bp localization (FITC). (C,G,K) Gal3 localization (Texas Red). (D,H,L) Merge of images.