Evaluation of the spontaneous onset of hematopoietic neoplasm in morgana^+/– mice. (A) Survival curves of morgana^+/+ and morgana^+/– mice. Kaplan-Meier analysis demonstrates a significant difference in survival (morgana^+/+: n = 21 and morgana^+/–: n = 25). (B) Representative picture of peripheral blood smears of diseased morgana^+/– mice. The scale bar = 10 μm. (C-D) White blood cell (WBC) count and hemoglobin (HGB) values in the peripheral blood of diseased morgana^+/– mice compared with healthy morgana^+/– and wild-type littermates. (E) Liver/body weight ratios (liver index) of morgana^+/–-diseased and wild-type mice. (F) Myeloid infiltration in the liver of morgana^+/–-diseased mice as confirmed by myeloperoxidase staining. The scale bar = 100 μm. (G) Spleen/body weight ratios (spleen index) of morgana^+/–-diseased and wild-type mice. (H-I) Flow cytometric analysis of (H) spleen and (I) BM single-cell suspension from diseased morgana^+/– and wild-type littermates and relative quantifications (n = 6 mice per group). (J) Flow cytometric analysis on the peripheral blood of representative morgana^+/– and morgana^+/+ donor, primary, and secondary recipient mice are shown. *P < .05, **P < .01, ***P < .001.