Molecular pathogenesis of morgana^+/– myeloproliferative disease. (A) Western blot analysis of BM protein extracts from morgana^+/+ and morgana^+/– mice before disease onset, stained with antibodies against morgana, ROCKI and II, phosphorylated MLC2 (P-MLC2), total MLC2, P-MYPT1, total MYPT1, P-AKT, total AKT, P-STAT5, total STAT5, and actin, used as a loading control. The graphs show the densitometric quantification of P-MLC2, P-MYPT1, and P-AKT bands normalized to total MLC2, MYPT1, and AKT (n = 3 mice per group). Note that ROCKI, ROCKII, and STAT5 phosphorylation were similar in morgana^+/+ and morgana^+/– BM. (B) Percentages of BM cells with >2 centrosomes in 8-month-old morgana^+/+ (n = 6) and morgana^+/– (n = 5) mice. (C) Percentages of BM cells with >2 centrosomes in morgana^+/–-diseased mice and morgana^+/+ littermates (n = 4 mice per group). (D) Percentages of aneuploid metaphase spreads obtained from the BM of morgana^+/+ and morgana^+/–-diseased mice (n = 4 mice per group). *P < .05, **P < .01.