LMP2A inhibited the E47 and PU.1 promoter activity through the ITAM motif. (A) Akata and BJAB cells were infected with pSIN, LMP2A, LMP2A-Y112F, and LMP2A-Δ74-85 expressing lentiviruses. After 3 days, the cells were infected with pCDH-GL3–, E47-, and PU.1 reporter–expressing lentiviruses. Luciferase activities were normalized with the GFP intensities of each transfectant. The activated fold was calculated by normalizing luciferase activities for the transfectant vs that for the pSIN with pCDH-GL3 vector control. (B) BJAB cells were infected with pSIN- and LMP2A-expressing lentiviruses. After 3 days, the cells were infected with pCDH-GL3–, E47-, and PU.1-expressing lentiviruses and incubated with dimethylsulfoxide (DMSO) or 20 μM LY294002 for another 48 hours. Luciferase activities from each transfectant were normalized with the GFP intensities. The activated fold for each reporter was calculated by normalizing luciferase activities for the transfectant vs that for the pSIN with pCDH-GL3 vector control. These data are a composite of 3 independent experiments (mean ± standard deviation).