PRMT5 transcript and miR96 are silenced in EBV-transformed cells and during B-cell immortalization. (A-B) PRMT5 messenger RNA (mRNA) expression was measured by qRT-PCR in resting B cells (RB), transformed B cells (A) and at various time points after EBV infection of normal B cells (B). C7M3 is a fully transformed LCL (B). The bar graph shows normalized fold expression of PRMT5 mRNA relative to normal B cells using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal control. (C-D) miR96 expression was measured by qRT-PCR in RB cells (RB), transformed B cells (C) and at various time points after EBV infection of normal B cells (D). JeKo is a MCL cell line used as control (C). The bar graph shows normalized fold expression of miR96 relative to normal B cells using GAPDH as internal control. **P < .01; ***P < .005. Error bars indicate SEM.