Platelet stimulation enhances plasminogen activation. (A) Washed platelets (5 × 10⁸ platelets/ml) were stimulated with CVX (100 ng/ml) + thrombin (100 nM) or TRAP-6 (15 µM) for 45 minutes. (B-C) Platelets were then diluted to a final concentration of 6 × 10⁷ platelets/ml ± tPA or uPA (1 nM) in the presence of hirudin (0.3 µg/ml) and D-Val-Leu-Lys-7-amido-4-methylcoumarin (0.35 mM). Plasmin generation was measured as fluorescence release and quantified as average FU per minute. **P < .01; ***P < .001; ****P < .0001 compared with unstimulated platelets. Data are expressed as mean ± SEM, n = 5. FU, fluorescence unit.