Figure 4
![Figure 4. Blood cell proliferation driven by mutant Idh. (A) Melanotic masses in third instar larvae from hml-Gal4, UAS-2xEGFP (hml>+), hml-Gal4, UAS-2xEGFP; UAS-Idh-WT [2;3] (hml>Idh-WT), and hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H [2;3] (hml>Idh-R195H). (B) Circulating GFP-positive hemocytes in hml>+, hml>Idh-WT, and hml>Idh-R195H larvae. (C) Quantitation of circulating hemocytes in hml>Idh-R195H larvae (n = 3; data are expressed as mean ± standard deviation). Drosophila were cultured at 29°C. Samples were visualized using a Nikon TE2000-E phase-contrast inverted epifluorescence microscope with a SensiCamQE camera, original magnification ×10, with MetaMorph, v6.2r6 software.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/125/2/10.1182_blood-2014-05-577940/4/336f4.jpeg?Expires=1735505989&Signature=SLCchcwz7S9aLa1-VqCqiwcNEoQfBBCISQjMOjBm9cJdUhOo0pbJ81ccflfZ30bwz9PWoPogMo1M8OiDvbaQYU81MQ3YfREobbPMELOKu2j10efhNw-GiGp1iEi467BGTFHNbwd4QuOo-hIvgVKLXUkv3rwDZ6lXAsJEjNLXy8J0GS3avSaTZeKthh50-GzVdgdNAuvZlxbXWZAVrLWxiJl3Rfm6XybrDepN4Bp1FWbKMzKHOhwBpabBbnbALq6JD9Y4T2b6GDu3AaK9f-IUwhBZ0QXWYKX7NPKsglCLuOmbBCVN0jgie3~boLKEtGamFk-7QsLMCn95UT83XmHaSQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Blood cell proliferation driven by mutant Idh. (A) Melanotic masses in third instar larvae from hml-Gal4, UAS-2xEGFP (hml>+), hml-Gal4, UAS-2xEGFP; UAS-Idh-WT [2;3] (hml>Idh-WT), and hml-Gal4, UAS-2xEGFP; UAS-Idh-R195H [2;3] (hml>Idh-R195H). (B) Circulating GFP-positive hemocytes in hml>+, hml>Idh-WT, and hml>Idh-R195H larvae. (C) Quantitation of circulating hemocytes in hml>Idh-R195H larvae (n = 3; data are expressed as mean ± standard deviation). Drosophila were cultured at 29°C. Samples were visualized using a Nikon TE2000-E phase-contrast inverted epifluorescence microscope with a SensiCamQE camera, original magnification ×10, with MetaMorph, v6.2r6 software.
