Spatiotemporal expression of zidh1 during zebrafish development. (A) The genomic loci surrounding human IDH1/2 in chromosomes 2q33 and 15q26.1 are syntenic with the regions of zidh1/2 in chromosomes 9 and 18. (B) Semi-qRT-PCR of zidh1 in different adult tissues (brain, kidney, spleen, heart, muscle, gill, liver, intestine, and skin) and at different embryonic stages normalized to β-actin. (C) Spatial expression of zidh1. Representative figures from at least 3 separate experiments containing more than 10 embryos per experiment are shown. Lateral view, anterior to the left, dorsal upwards. Dashed line indicates the level of the histological section in panel D. (D) Paraffin section at 36 hpf showing zidh1 expression around the ventral wall of the DA region (inset D′). (E) Mosaic fluorescent pattern (arrows) in embryos injected with linearized zidh1-gfp plasmid at 1-cell stage are almost completely abolished (lower panel) by zidh1^MO coinjection. (F) The percentages of embryos expressing gfp with or without zidh1^MO coinjection are enumerated. NC, notochord; CV, caudal vein. BA, brachial arches; CHT, caudal hematopoietic tissue. These data were performed in 3 independent experiments. Scale bars represent 250 μm.