zidh1 knockdown perturbed myeloid development. Expression of pu.1 mRNA (A,B,D) and protein (C) in control and zidh1^MO embryos at 12, 18, or 24 hpf. (C) Higher magnification of the yolk sac is shown in the insets. The numbers of embryos with the typical pu.1 immunostaining out of the total number of embryos evaluated are shown. mRNA expression of l-plastin (E), mpeg1 (F,F′), and mpo (G,G′) at 18 or 24 hpf. (H) Fluorescent microscopy of Tg(mpo:gfp) at 24 hpf showing decreased gfp⁺ cells after zidh1 knockdown. (I) Percentage of mpeg1:gfp⁺ and mpo:gfp⁺ cells in dissociated control and zidh1^MO embryos at 24 hpf by flow cytometry (3 independent experiments, representative results are shown in supplemental Figure 3). (J) The cell numbers of pu.1, mpeg1, and mpo positive cells at 24 hpf were counted; each symbol represents the cell number in 1 embryo. Scale bars represent 250 μm.