zidh1 knockdown perturbed definitive hematopoiesis. Expression of c-myb in control (upper panel), zidh1^MO embryos with (lower panel), or without (middle panel) zidh1 mRNA rescue at the ventral wall of the DA (black arrows) at 36 hpf (A) and at the caudal hematopoietic tissues (black arrows) at 48 hpf (B) and 4 dpf (C). The red arrows in panel C indicate c-myb expression in the thymus. (D) Expression of rag1 (red arrows) in control (upper panel), zidh1^MO embryos with (lower panel), or without (middle panel) zidh1 mRNA rescue at 4 dpf. These data were performed in 3 independent experiments. Scale bars represent 250 μm.