Overexpression of zidh1-R146H mutation-induced expansion of myeloid compartments. (A) Amino acid sequence alignment of IDH1 protein among human (h), mouse (m), and zebrafish (z), containing the cancer-associated residue R, which are marked by red box. *Residues involved in isocitrate binding. (B) Quantitation of 2-HG signal intensities relative to the glutamate signals of the same samples from 3 experiments. n.s., not significant. (C-F) Expression of pu.1 (C), l-plastin (D), mpo (E), and mpeg1 (F) in zidh1-WT-egfp– and zidh1-R146H-egfp–injected embryos at 24 hpf; the latter was treated with AGI-5198 (10 μM). The red arrows indicate myeloid cells on the yolk sac. The numbers of pu.1 (G), l-plastin (H), mpo (I), and mpeg1 (J) positive cells per embryos were manually counted. Scale bars represent 250 μm.