Aged T-bettg/tg mice spontaneously develop PAP-like disease. (A) Kaplan-Meier plot showing survival among mice with the indicated genotypes (n = 20 in each group). (B) Representative photomicrographs of the lungs of 50-week-old WT, T-bettg/wt, and T-bettg/tg mice. Alveolar spaces in T-bettg/tg mice are filled with acellular eosinophilic material and show inflammatory cell infiltration. H&E staining; bars = 100 µm. (C) Photomicrographs of lung sections from T-bettg/tg mice. The alveolar material is positive for PAS staining and for immunohistochemical staining of mouse SP-A. Bars = 100 µm. (D) Appearance of BAL fluid from 50-week-old WT, T-bettg/wt, and T-bettg/tg mice. (E) Levels of SP-A expression levels in lungs from 50-week-old WT, T-bettg/wt, and T-bettg/tg mice (n = 4 in each group). (F) The heat-map of genes whose expression changes are >10-fold between the genotypes in lung mRNAs. (G) Levels of T-bet mRNA expression levels in lungs from 50-week-old WT, T-bettg/wt, and T-bettg/tg mice (n = 4 in each group).
