Reorganization of BAL cell populations in T-bettg/tg mice. (A) Concentrations of IFN-γ, TNF, IL-6, MCP-1, IL-12p70, and IL-10 in the BAL fluid from WT, T-bettg/wt, and moribund T-bettg/tg mice at 30- to 50-weeks of age. n = 7, 3, or 5; **P < .01; *P < .05 (Kruskal-Wallis H test, followed by Steel-Dwass tests). (B) Numbers of macrophages, neutrophils, and lymphocytes in BAL fluid from 50-week-old WT, T-bettg/wt, and T-bettg/tg mice. Results are means ± SD; n = 3. **P < .01; *P < .05 (1-way ANOVA, followed by Tukey’s multiple comparisons tests). (C-D) Flow cytometry profiles for alveolar macrophages (C) and autofluorescence-positive population (D) among BAL cells gated to the leukocyte population (CD45+ cells). Data are representative of at least 3 mice. (E) Representative cytologies of BAL cells from mice with the indicated genotypes (Hemacolor staining; bars = 50 µm). (F) The heat-maps of M1- and M2-related genes. (G) Levels of Pparg, Abca1, Abcg1, and Bach2 mRNA expression levels in AF+ BAL cells. n = 3, 3, and 4.
