Number of myeloid cells are increased in the circulation of PAP mice. (A-D) Results of CBCs in PB from WT, T-bet^tg/wt, and T-bet^tg/tg mice at 25- to 30-weeks of age: WBCs (A), hemoglobin (B), hematocrit (C), and platelets (D). (E) Representative flow diagrams of living cell-gated populations in the PB of each mouse genotype. Myeloid cells were immunophenotypically defined as CD11b⁺. The myeloid-gated cells were further analyzed for their levels of Ly6C and Gr-1 expression. Inflammatory monocytes and neutrophils are defined as Gr-1^Mid, Ly6C^High and Gr-1^High, and Ly6C^Mid, respectively. (F-I) Numbers of myeloid cells (F), nonmyeloid cells (CD11b^-) (G), inflammatory monocytes (H), and neutrophils (I) in PB from each genotype; n = 3, 5, and 8. *P < .05 (1-way ANOVA, followed by Tukey’s multiple comparisons tests). HCT, hematocrit; HGB, hemoglobin; PLT, platelets.