Overexpression of T-bet in lymphocytes initiates PAP development. (A) Diagram of the experimental design. (B) Representative photomicrographs of the lung sections from Rag2^−/− recipients transplanted with splenocytes from WT, T-bet^tg/wt, or T-bet^tg/tg mice. H&E (top panels) and PAS (bottom panels) staining. Bars = 100 µm. (C) Changes in WBC counts in PB from Rag2^−/− recipients after transplantation of WT or T-bet^tg/tg splenocytes. (D-E) Changes in the numbers of inflammatory monocytes and neutrophils (D), and of T cells and B cells (E), in the PB of Rag2^−/− recipients after transplantation of WT or T-bet^tg/tg splenocytes. Symbols are means ± SD; n = 4 and 8. ***P < 0.001; **P < .01; *P < .05 (Student t test).