Figure 4
TET2 knockdown restores the JAK2V617F-induced impaired self-renewal capacity in vitro. Enumeration of colonies and serial replating capacity of 5 × 104 BM cells from recipients transplanted with 4 types of cells at 20 to 28 weeks after transplantation (n = 7-9). Compared to the BM cells from WT mice, those from JAK2V617F mice generated smaller numbers of colonies in vitro, whereas those from TET2KD mice generated larger numbers. Serial replating assays revealed restored serial replating capacity of cells by loss of TET2 in JAK2V617F BM cells. Error bars represent standard deviation. *P < .05 and **P < .01 vs WT mice; †P < .05 and ††P < .01 vs JAK2V617F mice.
