Effects of OB ablation on BM cellularity and stem and progenitor cell populations. (A) Total BM cellularity and (B) SP cellularity in control (TK−) and OB ablated (TK+) mice detected by flow cytometry. (C) Representative flow cytometry gating for LTHSCs. Percentage (left) and total cell number (right) of GMP in (D) BM and (E) SP of OB ablated and control mice. (F) Percentage and (G) total number of LTHSCs of OB ablated and control mice. (H) Percentage (left) and total cell number (right) of LSK Flt3⁻CD34⁻CD49b⁻CD229⁻ cells in MB and SP of OB ablated and control mice. Error bars represent mean ± SEM. Significance values: *P < .05, **P < .01, ***P < .001.