Engraftment of LTHSC from OB-ablated mice after transplantation. (A) Schematic representation of experimental procedure for transplantation of LTHSCs (CD45.2) selected from BM of OB ablated or nonablated mice into lethally irradiated CD45.1 wild-type recipient mice and secondary transplantation of whole BM cells from primary recipient mice into secondary recipient mice. (B) Engraftment of CD45.2 cells following transplantation of LTHSCs from control (TK−) or OB ablated (TK+) mice. (C) Engraftment of CD45.2 myeloid, B-cell, and T-cell lineages following transplantation of LTHSCs from control (TK−) or OB ablated (TK+) mice. (D) Lin⁻ cells selected from BM of control (TK−) or OB ablated (TK+) mice were stained with LTHSC markers, fixed, permeabilized, labeled with Ki-67 and DAPI, and analyzed for cell cycle by flow cytometry. Error bars represent mean ± SEM. Significance values: *P < .05, **P < .01.