Thrombosis after FeCl3-induced carotid artery injury occurs mainly via the contact system. Wild-type mice were given FXII, FVII, or control ASO by twice weekly subcutaneous injection for up to 6 weeks. A 1 × 2-mm filter paper presoaked in 10% FeCl3 was applied to the right carotid artery for 1.5 minutes to induce vascular injury (n = 6-10/group). (A) Blood flow was continuously monitored before and for 30 minutes after injury. Symbols represent the mean, and the bars reflect the SE. (B) Blood flow and TTO were determined in wild-type mice pretreated with FXII, FVII, or control ASO. TTO data are presented as a scatter diagram, where symbols represent individual values and horizontal lines represent the mean in each of the 3 groups. Values represent mean ± SD; *P < .001 compared with the control ASO group (ANOVA, Holm-Sidak method).
