Mn and C albicans exaggerated GVHD pathology. BMT were performed as shown in Figure 1A. (A) The representative images of the lungs stained with hematoxylin and eosin on day 21 after syngeneic (Syn) and allogeneic (Allo) BMT with or without Mn treatment are shown. Pathology scores of the lung (B), liver (C), and small intestine (D) from Syn controls (white bars), Mn-treated Syn mice (light gray bars), Allo controls (dark gray bars), and Mn-treated Allo mice (black bars) of 3 independent experiments were combined (n = 5 per group, means ± standard error). (E) Peripheral arterial oxygen saturation levels from 2 independent experiments are combined and means ± standard error are shown. (F-G) Allogeneic recipient mice were intraperitoneally injected with 1 × 105, 1 × 106, or 1 × 107 of heat-killed C albicans on the day of BMT. (F) The representative images of the lungs stained with hematoxylin and eosin on day 14 after allogeneic (Allo) BMT with or without the injection of 1 × 107C albicans are shown. (G) Bar graph shows pathology scores of the lung from Allo controls (dark gray bars) and Allo mice treated with indicated doses of C albicans (black bars) are shown. Data from 1 of 2 independent experiments were shown (n = 3-5 per group, means ± standard error). Original magnification ×200. Scale bars represent 100 μm (A,F). *P < .05; **P < .01.
