Bacterial lectin binding induced Ca²⁺ flux in the human cell line WSU-FSCCL under physiological Ca²⁺ conditions. (A) Flow-cytometric measurement of BCR surface expression on WSU-FSCCL and Ramos cell lines using anti-human IgM and anti-human κLC or λLC antibodies, respectively. (B) Surface binding profile of ConA to the indicated cells. (C) Western blot analysis of isolated surface proteins of the human cell lines subjected to glycosidase treatment as indicated. Size shift was analyzed by immunoblotting against anti-human μHC. (D) Binding profile of bacterial lectins to WSU-FSCCL and Ramos cell line cells measured by FACS. (E) Ca²⁺ mobilization of the indicated cell lines upon stimulation with bacterial lectins or anti-LC antibodies at the indicated concentrations. Data are representative of >3 independent experiments.