Fn-EDA+ interacts with platelet TLR4 to promote thrombosis, possibly through the platelet NF-κB signaling pathway. Platelet intracellular Fn-EDA+ is packaged from megakaryocytes and possibly also internalized via αIIbβ3 integrins, which are released upon activation from platelet α-granules. Fn-EDA+ may incorporate into fibrin clots, deposit on vessel wall, and induce NET formation.