Immune responses against TAAs in 114 HLA-A*02:01⁺ volunteer blood donors (cross-sectional study group I). CD8⁺ isolated T lymphocytes were coincubated with peptide-loaded irradiated T2 cells for 3 hours using 2 different peptide concentrations (0.1 and 10 µmol/L). Total RNA was isolated and qRT-PCR was performed. IFN-γ mRNA expression was normalized to CD8 mRNA levels and expressed as relative expression compared with stimulation with the irrelevant antigen gp100. (A) Peptide concentration of 0.1 µmol/L. (B) Peptide concentration of 10 µmol/L. The dashed lines indicate the threshold for positive immune responses (relative expression level of 2.0). Bars represent the median of a subgroup.