![Figure 1. MCL-1 is essential for recovery of the hematopoietic system from 5-FU challenge. (A) Experimental strategy. (B) Mcl-1+/−, Bcl-x+/−, and wild-type (control) mice were treated with 1 dose of 5-FU and survival was monitored over a 21-day period (median survival, Mcl-1+/− mice: 10 days; log-rank [Mantle-Cox] test, P < .0001). (C) RBC numbers in Mcl-1+/−, Bcl-x+/−, and wild-type mice at 10 days or when sick (Mcl-1+/−) after treatment with 5-FU. (D) Histologic analysis of the bone marrow of the mice from panels A and B at the same time point (representative images; ×40 objective, 250 µm scale bar). (E) RBC levels in the blood of 5-FU–treated Mcl-1+/−, Bcl-x+/−, and wild-type mice on the days indicated. n = 15 (wild type), 5 (Bcl-x+/−), 10 (Mcl-1+/−) mice; pooled results from 9 independent experiments. Data represent mean ± SEM. * and ** denote P < .05 or < .01, respectively, for the indicated comparisons (Student t test).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/125/21/10.1182_blood-2015-01-621250/4/3273f1.jpeg?Expires=1735529943&Signature=EJa~WiRobtW7AYa5e9nMRtT~ciasgR7ETGmrpGN8buK1wkMrN0r91vIyRRLBjxZ29iQmBJmFjUm3~KWLe0-jkkI5yWCss7S-~j~DNDVj9phwBBUVw5LG2Cp5Ah-d6n-7DmNJJU5HyjY6ozwNMLJ6bgh4cyGixAGqu8qMQjawNabqXFjchPpkIJCPuTRgwzxuD3NMY1NcpEsn7IDrEeh~52IM3PwX4j4j6rw80pJqNH0ErBZngAmeWsabxYtIfpnHl84hcBQYCTInUh9BU3l50rYSq9FxPM3F7dJmOCYyi4Mft7fKBl~WjS36jASMkEmouzfYM7DnfG7LYrRaW4khUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MCL-1 is essential for recovery of the hematopoietic system from 5-FU challenge. (A) Experimental strategy. (B) Mcl-1+/−, Bcl-x+/−, and wild-type (control) mice were treated with 1 dose of 5-FU and survival was monitored over a 21-day period (median survival, Mcl-1+/− mice: 10 days; log-rank [Mantle-Cox] test, P < .0001). (C) RBC numbers in Mcl-1+/−, Bcl-x+/−, and wild-type mice at 10 days or when sick (Mcl-1+/−) after treatment with 5-FU. (D) Histologic analysis of the bone marrow of the mice from panels A and B at the same time point (representative images; ×40 objective, 250 µm scale bar). (E) RBC levels in the blood of 5-FU–treated Mcl-1+/−, Bcl-x+/−, and wild-type mice on the days indicated. n = 15 (wild type), 5 (Bcl-x+/−), 10 (Mcl-1+/−) mice; pooled results from 9 independent experiments. Data represent mean ± SEM. * and ** denote P < .05 or < .01, respectively, for the indicated comparisons (Student t test).
