Complete rescue of the Mcl-1^+/− hematopoietic repopulation defects by PUMA deficiency. (A) Survival of Mcl-1^+/−;Puma^−/−, Mcl-1^+/−;Bim^−/−, Mcl-1^+/−, and wild-type mice after treatment with 1 dose of 5-FU; wild-type and Mcl-1^+/− animal survival curves are reproduced here from Figure 1 to aid comparison. (B) Numbers of reticulocytes and RBCs in the blood of the mice described in panel A; values from mice reconstituted with wild-type bone marrow cells are reproduced here from Figure 1 to aid comparison. (C) Bone marrow (test) cells from wild-type, Mcl-1^+/−, Mcl-1^+/−;Bim^+/−, Mcl-1^+/−;Bim^−/−, Mcl-1^+/−;Puma^+/−, and Mcl-1^+/−;Puma^−/− mice (all C57BL/6-Ly5.2) were mixed 1-to-1 with competitor C57BL/6-Ly5.1 bone marrow cells and injected into lethally irradiated C57BL/6-Ly5.1 recipients (R1). After 16 weeks, the percentages of test vs competitor-derived LSK stem/progenitor cells in the bone marrow of R1 reconstituted mice were determined by flow cytometric analysis. R2 was achieved as described in Figure 3A. After 16 weeks, percentages of test- vs competitor-derived LSK stem/progenitor cells in the bone marrow of R2 reconstituted mice were determined by flow cytometric analysis. Data from animals reconstituted with wild-type and Mcl-1^+/− bone marrow cells are reproduced from Figure 3E to aid comparison with cells from the other genotypes. (A-B) n = 4 mice. Pooled results from 2 independent experiments are shown. (C) n = 4 biological samples (each circle represents the mean of 3 replicate mice). Pooled results from 9 independent experiments are shown. Data represent mean ± SEM. *, **, ***, **** denote significant differences where P < .05, < .01, < .001, < .0001 between the indicated groups (Student t test).