A representative sensogram (Biacore analysis) showing A2 domain dissociation from FVIIIa after activation of FVIII by thrombin. The top solid line represents D519V/E665V; the dashed line represents BDD-FVIII. The lower lines (dashed and solid) are the respective nonspecific antibody control channels. All data have been subtracted from a blank channel and are representative of 2 independent experiments.