Transgenic expression of Fbxl10 in mice develop leukemia. (A) Kaplan-Meier survival plots for the Fbxl10 Tg (Tg) (n = 30) and control WT (Ctrl) (n = 20) mice. (B) Representative peripheral blood smear (left) and macroscopic appearance (right) of a leukemic Tg mouse. Proliferation of blast cells and massive hepatosplenomegaly are shown. (C) Flow cytometric profiles in the leukemic tissues of 2 Tg mice. TG-1 was positive for Mac1 and/or Gr1 positive but negative for Thy1.2 and B220 and diagnosed with AML, whereas TG-2 was positive for B220 but negative for Thy1.2, Gr1, and Mac1 and accordingly diagnosed with B-lymphocytic leukemia (B-ALL). (D) Survival curves of Tg, Tg/MOL4070LTR (Tg mice infected with MOL4070LTR), control (Ctrl), Ctrl/MOL4070LTR. MOL4070LTR infection induced acute leukemias in all Tg/MOL4070LTR mice and 1 Ctrl/MOL4070LTR mouse. The Tg/MOL4070LTR mice showed significantly accelerated mortality compared with the original Tg mice, whereas WT Ctrl mice did not. The time of MOL4070LTR injection is indicated by an arrow. (E) Survival curves of Tg, Tg/ENU (Tg mice injected with ENU), Ctrl, Ctrl/ENU mice. ENU-induced acute leukemias in all Tg/ENU and 1 Ctrl/ENU mice. The Tg/ENU mice showed significantly accelerated mortality compared with the original Tg mice, whereas Ctrl mice did not. The time of ENU injection is indicated by an arrow. For survival comparisons, the Tg and control cohorts shown in panel A were merged in panels D and E.