Ly6A/Sca-1 promoter-driven Fbxl10 overexpression induces cell-autonomous proliferation in HSCs. (A) Absolute numbers of HSCs (LT-HSC, ST-HSC, and MPP) and progenitor (CLP, CMP, GMP, and MEP) cells (n = 4-8, *P < .025). Surface markers to separate mouse stem and progenitor cell subsets are shown in supplemental Table 3. (B) Increased in vivo BrdU incorporation in LT-HSCs of Tg mice. BrdU-positive cells in LT-HSC and ST-HSC were analyzed by flow cytometry (n = 4 per group, *P = .024). (C) Summary of flow cytometric pyronin Y analysis of CD34⁻ LSK or CD34⁺ LSK fractions in control or Tg BM cells (n = 4 per group). Error bars, SD. (D) Percent chimerism of donor cells in the peripheral blood after serial BM transplantation. Upper panel shows a schematic diagram of the experimental strategies. Each dot shows the percent chimerism of donor cells in the peripheral blood of recipients 12 weeks after the secondary transplant. Bars indicate the average (n = 6 per group, *P < .05, **P < .01). CBC, complete blood cell count; MEP, megakaryocyte/erythroid progenitor; n.s., not significant; SD, standard deviation; WBC, white blood cell count.