Tet2 loss confers enhanced self-renewal to Jak2V617F HSPCs in vitro and in vivo. (A) Colony-forming unit assays from unfractionated bone marrow derived from WT, Jak2^VF, Tet2^null, and Jak2^VF/Tet2^null mice. Results represent the average of triplicate assays (mean ± SEM). The percentage of 45.2 donor chimerism assessed in peripheral blood (PB) total WBCs (B), PB Gr1⁺ cells (C), and PB B220⁺ cells (D) from lethally irradiated secondary recipients of Jak2^VF, Tet2^null, or Jak2^VF/Tet2^null LSK cells competed against an approximately equal number of 45.1 WT LSK cells, measured 4 to 17 weeks posttransplantation (mean ± SEM; n = 5 in each group). The percentage of 45.2 donor chimerism assessed in whole bone marrow (BM) cells (E), whole spleen (SPL) (F), and LSK BM cells (G) from lethally irradiated secondary recipients of Jak2^VF, Tet2^null, or Jak2^VF/Tet2^null LSK cells competed against an approximately equal number of 45.1 WT LSK cells, measured 18 weeks posttransplantation (mean ± SEM; n = 5 in each group). P values for each of the comparisons are indicated in the figure.