Analysis of murine megakaryocyte ploidy. (A-B) The numbers and distribution of megakaryocytes with various amounts of DNA content after 7-day culture in the presence of thrombopoietin from KO and TG mice, as indicated, are shown. (C) The quantitation of the ploidy distribution (ie, the percentage of cells with 2N, 4N, 8N, and 16N) is shown as the means and standard deviation from 4 independent experiments. ANOVA was performed and revealed no statistical significance among the groups (P > .05).