Agonist-induced platelet aggregation and release of rADAMTS13 from transgenic platelets. (A-C) Aggregation tracing recorded using a light scattering aggregometer for washed platelets isolated from various mice, as indicated, after being stimulated with soluble type 1 collagen (2 µg/mL), α-thrombin (0.1 U/mL), and 2MesADP (30 nM), respectively. (D) Western blotting demonstrates the rADAMTS13 (arrowheads) released from TG platelets after stimulation with a buffer alone (CON), type 1 collagen [Coll.(10 µg/ml)], α-thrombin [Throm. (1 U/ml)], and 2MesADP (ADP, 100 nM) as indicated in each lane. (E) The residual rADAMTS13 (top) and control β-actin (bottom) are detected in platelet lysate after aggregation. (F) The ratios of the residual rADAMTS13 (top band) to β-actin in platelet lysate (the means ± standard deviation) (n = 3) are shown. ANOVA was used to determine statistical significance of the differences among various groups. *P < .05; **P < .01.