IL8 is overexpressed in stem and progenitor cells in MDS and AML. (A) Gene expression data from sorted AML/MDS bone marrow samples were compared with healthy controls and (B-D) revealed significantly increased IL8 expression in LT-HSCs (Lin-ve, CD34⁺, CD38⁻, CD90⁺, N = 12 AML/MDS, HC = 4), ST-HSCs (Lin-ve, CD34⁺, CD38⁻, CD90⁻), and GMP (Lin-ve, CD34⁺, CD38⁺, CD123⁺, CD45RA⁺) (P < .001, FDR < 5%). Cytogenetic abnormalities are depicted as follows: NK, normal karyotype; CK, complex karyotype; −7 = deletion of chr7. Representative flow plots show sorting strategy for (E) healthy control and (F) AML sample. (G) qRT-PCR on sorted cells reveals increased expression of IL8 in all AML/MDS HSCs (LT/ST) and GMP.