No upregulation of Cebpa is observed in ΔCF/ΔCF peritoneal mast cells. (A) Representative FACS data for markers of mast cells (c-Kit and FcεRIα) and basophils (CD11b and CD49b) on ΔCF/ΔCF PMCs. PMCs were cultured ex vivo for 14 days and then were cultured in the absence (−) or presence (+) of 4-OHT for an additional 9 days. The average percentages and SD of the DP cells within the gates are shown. N = 4. (B) The results of the Q-PCR analyses of the indicated genes in PMCs and BMMCs prepared from wild-type mice (WT) and ΔCF/ΔCF PMCs cultured in the absence (−) or presence (+) of 4-OHT. *P < .05; **P < .01 [compared with data from 4-OHT(−) ΔCF/ΔCF PMCs]. N = 4. (C-D) Schematic summaries of the present study. (C) Summary of the reprogramming activity of ΔCF/ΔCF BMMCs. (D) Summary of the phenotypes of the genetically manipulated BMMCs and PMCs used in this study.