Recombinant H-Y protein microarray analysis. (A) Temporal changes of overall HY-Ab development throughout 3 years post-HCT. The various colors indicate relative antibody quantification as a factor of each HY-Ab–seropositive threshold. White squares indicate that samples were unavailable. Black and brown denote death and relapse, respectively. From left to right, the columns represent 2, 3, 6, and 9 months, 1 year, and >1 year posttransplant. UTY2 and SMCYb are shown as representatives of 3 UTY and 6 SMCY fragments, respectively. (B) Changes in prevalence of HY-Abs throughout 3 years post-HCT. UTY2 and SMCYb are shown as representatives of 3 UTY and 6 SMCY fragments, respectively. m, months.