miR-24 regulates VWF maturation and secretion. (A) Endothelial cells were transfected with miR-24 mimic or control construct. The mRNA levels of ADAMTS13, FURIN, ENOS, TSP-1, HRH1, and CD62P in cell lysates were analyzed by qPCR (normalized to GAPDH and then normalized to mimic control). *P < .05. (B) Endothelial cells transfected with miR-24 inhibitors or control constructs. The mRNA levels of ADAMTS13, FURIN, ENOS, TSP-1, HRH1, and CD62P in cell lysates were analyzed by qPCR (normalized to GAPDH and then normalized to inhibitor control); *P < .05. (C) VWF protein levels (VWF, TSP-1, p-eNOS, eNOS, Furin, and HRH1) in cell lysates determined by western blot. Actin and GAPDH were used as controls. (D) Endothelial cells transfected with miR-24 controls (upper) or inhibitors (lower). Cell surface attached VWF in endothelial cells was visualized by confocal microscopy (DAPI, blue; VWF, green) after 50 µM histamine stimulation (2 minutes before preparation for microscopy).