018Z human pre-B-ALL cells induce isolated CNS leukemia in NOD/SCID mice and combined CNS/peripheral leukemia in NSG mice. (A) A Kaplan-Meier survival curve of NOD/SCID (n = 10) and NSG (n = 4) mice injected intravenously with 018Z cells (P = .001; log-rank test). (B) Representative hematoxylin and eosin staining of brain sections from NOD/SCID (lower; n = 10) and NSG (upper; n = 3) mice presenting leukemia infiltration in the subarachnoid space. (C) Flow cytometry analysis of peripheral leukemia percentage in the (i) blood, (ii) BM, and (iii) spleen using antibodies for CD10 and CD19. Values are means ± SE (P < .0001; unpaired t test). (D) Representative pictures of spleens taken from (i) NSG or (ii) NOD/SCID mice.