Viral antigen expression variation is comparable in immunodeficient and immunocompetent hosts. (A) Analysis of polyadenylated RNA-seq data confirms that viral expression is detectable in HIV-negative sBL in immunocompetent hosts. Expression of latency I associated EBNA1 is robustly detected in primary samples (prefixed by SLN) and cell lines. (B) LMP1 and LMP2 RNA were detected in most primary HIV-negative sBL tumor samples. SLN2521 is excluded in the right panel due to low read counts. (C) Viral RNA is translated into immunogenic protein expression in a subset of cases (additional analysis in Table 3). IHC stains in 4 representative cases demonstrate that ARL (left) and sBL (right) cases contain highly immunogenic viral proteins. All samples are positive for the respective stains.