Increased B cell maturation and class switched immunoglobulins in reconstituted NSGAb°DR1 mice. (A) Representative flow cytometry dot plots of splenic B cells gated on CD45⁺CD19⁺ (left) and quantified (right) from 3 independent experiments with n ≥ 5 per group. (B) Serum immunoglobulin levels quantified by ELISA. Each data point represents an individual mouse from 3 independent experiments. Solid bars are the mean ± SEM. Statistical analysis was performed using multiple t test corrected for multiple comparisons using the Holm-Sidak method (A), Mann-Whitney test (B). *P < .05, **P < .01, ***P < .001.