Constitutively active NF-κB in eosinophils increases their survival during N brasiliensis infection. (A) Percentage of RFP⁺ cells among eosinophils (Siglec-F⁺SSC^hi) in naive (left) and day 9 N brasiliensis–infected (right) EpxCre/R-RFP/IκB^F/+ (gray bars) and EpxCre/R-RFP/IκB^F/F (black bars) mice. Bars show the mean + SEM from 6 to 9 mice per group from pooled experiments. *P < .05; **P < .01. (B) Number of total (left) and RFP⁺ (right) eosinophils in the spleen of EpxCre/R-RFP/IκB^F/+ (gray bars) and EpxCre/R-RFP/IκB^F/F (black bars) mice on day 10 after N brasiliensis infection. Bars show the mean + SEM from 3 to 6 mice per group from 3 pooled experiments. (C) Expression of CD62L on RFP⁺ and RFP⁻ eosinophils from indicated mice. Dot plots are representative of 3 analyzed mice per group. (D) Dot plots show frequencies of late apoptotic eosinophils (Siglec-F⁺SSC^hiRFP⁺annexinV⁺PI⁺) in naive and day 9–infected EpxCre/R-RFP/IκB^F/+ (gray bars) and EpxCre/R-RFP/IκB^F/F (black bars) mice. Bar graph shows the mean + SEM of late apoptotic Siglec-F^hiRFP⁺ eosinophils from 6 naive and infected mice per group. *P < .05. BAL, broncho-alveolar lavage; SI, small intestine.