Figure 6
The Bcl-xL inhibitors HA14.1 and BH3I-1 promote apoptosis of murine and human eosinophils. (A) Murine BMDEs were cultured in presence of IL-5 and the indicated Bcl-xL inhibitors for 4 hours (black bars) or 24 hours (gray bars) and analyzed for apoptosis by flow cytometry. Dot plots show representative samples at 24 hours after addition of the inhibitors. (B) Human eosinophils isolated from the peripheral blood of healthy volunteers were cultured for 24 hours in the presence of IL-5 and the indicated Bcl-xL inhibitors. Bars show the mean + SEM (n = 3) of 3 independent experiments.
