FL-associated STAT6 mutations are intrinsically activating and partially STAT6-Y641 independent. Results of STAT6 luciferase assays in HEK293T cells and corresponding immunoblots. (A) Luciferase assay results in HEK293T cells indexed to the results for unstimulated STAT6 wt measurements; IL-4 stimulation is indicated. (B) Immunoblot results for the experiment presented in (A) using antibodies against STAT6, p-STAT6-Y641, and actin. (C) Luciferase assay results in HEK293T cells indexed to the results for unstimulated STAT6 wt measurements; IL-4 stimulation is indicated. (D) Immunoblot results for the experiment presented in (C) using antibodies against STAT6, p-STAT6 Y641, and actin.