Viral reactivation and T-cell reconstitution in CMV-reactivating and nonreactivating patients. (A) CD8⁺ Tem counts at day +365 posttransplant. (B) CMV viral load measured longitudinally. Each CMV-reactivating patient is shown as a unique colored line. Donor/recipient (D/R) pretransplant CMV serostatus is also indicated. Also shown is the mean day of CMV reactivation (±standard error of the mean [SEM]), depicted as a purple bar. (C) Longitudinal analysis of WBC, granulocytes, NK cells, monocytes, lymphocytes, total T cells, and total B cells posttransplant. Data are mean ± SEM in +CMV patients (red traces, n = 7), −CMV patients (blue traces, n = 10), and healthy controls (green diamond longitudinally extended as gray area, n = 10). *P ≤ .05; Wilcoxon rank-sum test. (D) Longitudinal analysis of absolute numbers ± SEM of CD4⁺ and CD8⁺ T-cell reconstitution (+CMV [n = 7] and −CMV [n = 10]). Also shown are the means ± SEM of 10 healthy controls (green diamond extended longitudinally as gray area). (E) Analysis of CD4:CD8 ratio at baseline and days 100, 180, and 365 posttransplant as mean ± SEM in +CMV, −CMV, and healthy controls (green diamond). *P ≤ .05; **P ≤ .01; ***P ≤ .001; NS, nonsignificant, Wilcoxon rank-sum test. (F) Analysis of CD4:CD8 ratio at day 100 of an extended cohort of 46 HSCT patients: +CMV (n = 26) and −CMV (n = 20).