Longitudinal analysis of CD8 naive and memory T-cell subpopulation reconstitution in +CMV and −CMV patients. (A) Representative flow cytometry analysis of memory subset marker expression in CD8⁺ T cells. Cells were gated as follows: lymphocytes were identified by FSC and SSC, CD14⁻CD3⁺ T lymphocytes were identified, further distinguished into CD8⁺ and CD4⁺ T cells, and analyzed for their expression of CCR7 and CD45RA memory markers. Tnaive were identified as CCR7⁺/CD45RA⁺, TCM: CCR7⁺/CD45RA⁻, TEM: CCR7⁻/CD45RA⁻, TEMRA: CCR7⁻/CD45RA⁺. (B-C) Longitudinal analysis of CD8⁺ naive and Tem subsets depicted in percentage frequency (B) or absolute cell numbers (C). Data are mean ± SEM in +CMV patients (n = 7), −CMV patients (n = 10), and healthy controls (n = 10). Also shown is the mean day of CMV reactivation (±SEM), depicted as a purple bar. (D) Left, Representative flow cytometry analysis at day +365 shows CD28 expression (left panel) and Granzyme B expression (right panel) on CD8⁺ Tem. Right, Longitudinal analysis of mean ± SEM CD28⁻CD8⁺ Tem (left y-axis) and Granzyme B⁺CD8⁺ Tem (right y-axis). (E) Longitudinal analysis of PD-1⁻/CD57⁺CD8⁺ Tem (solid lines) and naive T cells (dotted lines) of +CMV (n = 7), −CMV (n = 10) patients, and healthy controls. All data are mean ± SEM. *P ≤ .05; **P ≤ .01 Wilcoxon rank-sum test. CCR, C-C chemokine receptor; FSC, forward scatter; SSC, side scatter; TCM, central memory T cells; TEMRA, effector memory-RA T cells.