Inter- and intrapatient evaluation of shared TCRβ clones between purified Tem and tetramer⁺ cells. (A) Color-coded pairwise TCRβ clone sharing between samples. Colors range from yellow (indicates no sharing) to blue (indicates 100% sequence sharing). This matrix depicts the degree of clone sharing between all identified TCRβ sequences in the sorted Tem and tetramer⁺ cells. (B) Enrichment of Tetramer⁺ cells in the clonally expanded Tem. Shown are dot plots for 4 +CMV patients which depict the binned frequencies for each of the TCR clones in the sorted CD8⁺ Tem (“TEM”; black) and sorted tetramer⁺ cells (“TETPOS”; green). These graphs depict the change in frequencies of productive clones, each compared with a sample-specific median frequency value. Bins represent 0.25 log(10) intervals, with binned data reported using a log₁₀ scale. Tem clones are shown as black circles and the Tetramer⁺ clones are shown as green circles.