Impact of CMV reactivation and GVHD on the reconstitution of CD4⁺ Tnaive and CD31⁺ recent thymic emigrants. (A) Longitudinal analysis of the reconstitution of CD4⁺ Tnaive. Data are mean ± SEM in +CMV patients (n = 7), −CMV patients (n = 10), and healthy controls (n = 10). Also shown is the mean day of CMV reactivation (±SEM), depicted as a purple bar. (B) Longitudinal analysis of the reconstitution of CD31⁺/CD4⁺ recent thymic emigrants. Data are mean ± SEM in +CMV (n = 7), −CMV patients (n = 10), and healthy controls (n = 10). *P ≤ .05; **P ≤ .01; NS, nonsignificant; Wilcoxon rank-sum test. (C) Longitudinal analysis of the reconstitution of CD4⁺ Tnaive dichotomized based on GVHD status. Data are mean ± SEM in +GVHD patients (purple circles, n = 10), −GVHD patients (green squares, n = 7), and healthy controls (green circle and gray bar, n = 10). (D) Longitudinal analysis of the reconstitution of CD31⁺/CD4⁺ recent thymic emigrants dichotomized based on GVHD status. Data are mean ± SEM in +GVHD patients (purple circles, n = 10), −GVHD patients (green squares n = 7), and healthy controls (green circle and gray bar, n = 7). (E) Longitudinal analysis of the reconstitution of naive CD8⁺ T cells dichotomized based on GVHD status. Data are mean ± SEM in +GVHD patients (purple circles, n = 10), −GVHD patients (green squares, n = 7), and healthy controls (green circle and gray bar, n = 10).