Immunohistochemistry for CD235a (glycophoryin) and Hb in human renal biopsies. Fluorescence immunohistochemistry in human normal peritumoral control (A) and SCD (B-G) kidney sections (n = 2) reacted with anti-human Hb and anti-human CD-235a IgG. High magnification micrographs were taken from tubulo-interstitial area (×600; white bars = 10 μm). White arrows identify CD235a⁺ or Hb+ particles. White circles surround a double-stained MPs. Note the submembrane deposition of Hb within SCD erythrocytes (C-E). (F) Immunohistochemistry with anti-human Hb IgG (×400). Black arrows identify counterstained capillary endothelial cell nuclei. Green arrows show Hb-positive cell fragments, and red arrowheads point at intact erythrocytes. Red stain reveals Hb in intact erythrocytes or deposited at the surface of nucleated vascular wall cells. (G) Fluorescence immunohistochemistry (×600) showing vascular Hb deposits.