Demonstration of vessel fibrin, platelets, and permeability after contact activation injury to the lung. (A) Immunofluorescence of a large vessel with an internal fibrin rim using Mab 59D8 (green) and platelet thrombus using anti-CD42c (red) with a fibrin border after Col/Epi-induced pulmonary embolism. (B) Immunofluorescence of microvessel thrombi for fibrin after Col/Epi pulmonary embolism. (C) Immunofluorescence of colocalization of fibrin and platelets in microvessel thrombi after Col/Epi pulmonary embolism. (A-C) Images were obtained on a Nikon Eclipse TE2000-S microscope at ×10 magnification. (D) Representative whole-lung Evans blue dye permeability in Klkb1^+/+ and Klkb1^−/− tissue. The images were taken with an iPhone 5 through a ×2 objective of a Nikon C-W 10XB/22 dissecting microscope. (E) Vascular permeability score for Evans blue dye in Klkb1^+/+ (n = 5) and Klkb1^−/− (n = 4) lungs (P < .036).