Loss of podoplanin in the neural tube causes cerebral hemorrhages. (A) Pdpn^fl/flNes-Cre embryos develop hemorrhages in the brain by E12.5 (n = 4), whereas Pdpn^fl/fl littermates appear normal (n = 3). Scale bars, 1 mm. (B) H&E coronal sections from E12.5 embryo heads of Pdpn^fl/fl (n = 3) and Pdpn^fl/flNes-Cre (n = 4) embryos. White boxes in the middle panel magnify areas in the bottom panel. Yellow arrows indicate reticulated red blood cells in the ventricles, and yellow asterisks (*) marks an area of hemorrhage in the neuro-epithelium. Scale bars, 50 μm.